Different degrees of lever arm rotation control myosin step size

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چکیده

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Different degrees of lever arm rotation control myosin step size

Myosins are actin-based motors that are generally believed to move by amplifying small structural changes in the core motor domain via a lever arm rotation of the light chain binding domain. However, the lack of a quantitative agreement between observed step sizes and the length of the proposed lever arms from different myosins challenges this view. We analyzed the step size of rat myosin 1d (M...

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Myosin VI lever arm rotation: fixed or variable?

Two recent articles addressed the power-stroke of myosin VI molecules during stepping. Although both groups measured the angles of fluorescent probes attached on the myosin VI molecule lever arm using polarized fluorescence techniques, they differ about whether the myosin VI lever arm rotation is fixed or variable. Here we discuss the causes of the discrepancy between the two studies and the im...

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Myosin VI (MVI) is the only known member of the myosin superfamily that, upon dimerization, walks processively toward the pointed end of the actin filament. The leading head of the dimer directs the trailing head forward with a power stroke, a conformational change of the motor domain exaggerated by the lever arm. Using a unique coarse-grained model for the power stroke of a single MVI, we prov...

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ژورنال

عنوان ژورنال: Journal of Cell Biology

سال: 2003

ISSN: 1540-8140,0021-9525

DOI: 10.1083/jcb.200212039